How to Activate a Freeze-Dried (Lyophilised) Peptide: A Quick Guide

Freeze-dried (or lyophilised) powders are a popular form for many supplements, peptides, and pharmaceuticals. This form helps preserve the stability and shelf life of sensitive compounds. But before use, the powder must be reconstituted—meaning it needs to be activated with a liquid. Here's how to do it safely and effectively.

What You'll Need

• Sterile water (or bacteriostatic water, depending on the product and usage)
• Alcohol swab
• Syringe (usually 1–3 mL, depending on your needs)
• Vial containing the lyophilised powder

Step-by-Step Instructions

1. Clean your workspace
   Choose a clean, well-lit area and wash your hands thoroughly. Disinfect the vial cap and water vial with an alcohol swab.
2. Draw up the liquid
   Use a sterile syringe to draw up the recommended amount of water. The volume will depend on the product—common volumes are between 1 and 2 mL.
3. Insert the syringe into the powder vial
   Insert the needle into the vial at an angle to avoid too much pressure build-up. Slowly inject the liquid down the side of the vial, not directly onto the powder. This helps prevent foaming or damaging the compound.
4. Let it dissolve
   Gently swirl the vial (do not shake it) to help the powder dissolve. This may take a minute or two. Once fully dissolved, the liquid should be clear and free from particles.
5. Storage and use
   Depending on the compound, the reconstituted solution may need refrigeration and should be used within a specified timeframe. Always follow the product’s storage instructions.

Final Tips

• Only use the recommended type of water for reconstitution.
• Never reuse syringes or mix different products in the same vial.
• If the solution looks cloudy or contains particles after mixing, do not use it.

Activating your lyophilised powder properly ensures its effectiveness and safety. If in doubt, always consult with a qualified professional or the product manufacturer.